Journal of Parasitology Research

Background. The worldwide distribution and occurrence show that more than 1.8 billion people, accounting for 28% of the global population, are infected with A. lumbricoides infection due to poor hygiene. The World Health Organization aims to eliminate morbidity from A. lumbricoides infection in children by 2030 by at least 75%. Surveys in the Pwani Region of Tanzania have shown poor hygienic risk factors due to high population and lack of sufficient toilets/latrines, poor sewage waste disposal, and insufficient clean drinking water that encourages the reproduction, transmission, and reinfection of A. lumbricoides. No study has been conducted in Pwani Region to determine the extent of A. lumbricoides infection among the population. This research tends to explore more about the status of A. lumbricoides infection in Kibaha District in the Pwani Region, Tanzania. Methods. A cross-sectional study was done on 400 children (170 males and 230 females) who were under the age of 5 years old at Kongowe Health Centre. Collected data for A. lumbricoides infection measured were analyzed using Microsoft Excel, while data for sociodemographic findings and risk assessment were subjected to bivariate and multivariable analysis using Statistical Package for Social Sciences version 28.0.1.0 software (SPSS Inc., USA) at the level of significance of . Results. Of the 400 children who participated in the laboratory testing of A. lumbricoides infection, 194 were found positive for A. lumbricoides infection. Amongst the 194 children who were found to be positive, 4.1%, 38.7%, and 57.2% were found to be in the age group of below 1 year old, 1 to 2, and 3 to 4 years old, respectively. The tabulated responses on the social demographic responses showed that 81.8% of the responses at and 80.6% at knew that A. lumbricoides infection is acquired from faeces and uncooked food, respectively, as 61.8% at and 63.2% at respondents did not accept that soil and dirty water, respectively, were not sources of A. lumbricoides infection. At and , 64% and 91.2% of the respondents showed the importance of using latrines/toilets and washing fruits, respectively. Further results showed that 69.8% and 37.6% of the respondents reacted with no importance of washing hands with soap after using a toilet/latrine and no need to treat or boil water before drinking, respectively. Conclusions. A. lumbricoides infection has a high prevalence in infants of the Kibaha District of Tanzania, especially in Kongowe village. The infection is dominant in males compared to female children. The prevalence is due to risk factors such as inadequate sanitation, low level of education, knowledge, and awareness of prevention strategies.

Haemonchus contortus is a blood sucking parasite resulting a massive economic loss in tropical and subtropical sheep rearing areas. A cross-sectional study was conducted to estimate the prevalence of haemonchosis and its predictors at Bahir Dar municipal abattoir, northwestern Ethiopia, from December 2022 to April 2023. A total of 378 abomasum of sheep were taken by using systematic random sampling and inspected according to standard procedures. Accordingly, the findings of this study revealed an overall prevalence of 34.40% (30/378) (95% CI: 29.75-39.35). The presence of H. contortus in female sheep (46.41%) was significantly higher than in male (23.35%) (). The same is true; prevalence of haemonchosis in young (<1 year) sheep was 43.33% significantly () higher than that of adult (≥1 year) which was 26.26%. Based the origin of the animals in the current study, it was nonsignificant variation (). The current study depicted that H. contortus infection is a common parasitic disease and requires remarkable attention to the prevention and control of haemonchosis at the study area.

Background. Despite extensive efforts and achievements made in prevention and control, malaria is still a public health problem in Ethiopia. Currently, the case number has been climbing, even changing the epidemiology to the previously low-burden reporting locations. Therefore, our study is aimed at assessing malaria prevalence and associated risk factors in outpatients, Northwest Ethiopia. Methods. A facility-based cross-sectional study was conducted among 318 malaria-suspected outpatients from October 22 to December 15, 2022, at Teda Health Center, Northwest Ethiopia. Sociodemographic characteristics and malaria risk factors were assessed by a face-to-face interview using a pretested questionnaire. Plasmodium parasites were detected by using microscopy on a blood film stained with 10% and 3% Giemsa stain. The data were analyzed using Statistical Package for Social Sciences software version 25, and statistical significance was reported at a value < 0.05. Results. Of the 318 study participants, 124 (39.0%; ) tested positive for Plasmodium infection. Plasmodium vivax, P. falciparum, and mixed infections were identified in 71 (57.3%), 47 (37.9%), and 6 (4.8%), respectively, among positive study participants. Individuals above the age of 15 (, 95% CI 1.370-5.336) were more likely to be infected with Plasmodium than under-five-year-old individuals. Rural residents (, 95% CI 1.281-5.098), those who sleep or work outside at night (, 95% CI 1.020-3.875), and individuals who did not use long-lasting insecticide-treated net (, 95% CI 1.633-6.499) and had a family member with a history of malaria (, ) had higher odds of acquiring Plasmodium infection than their counterparts. Conclusion. In our study area, malaria was a major health problem, with P. vivax being the dominant species. Multiple environmental and behavioural factors were associated with the infection acquisition. Therefore, urgent holistic intervention is required.

Malaria is one of the major public health problems in sub-Saharan Africa, including Ethiopia. Children under the age of five are immunologically naive to plasmodium parasites, making them the most vulnerable group to clinical manifestations of malaria infection. Despite the severity of the disease in children under five years of age, most studies on malaria focus on the adult population. In the present study, a cross-sectional study design based on health facilities was used during the minor malaria season from February 18 to May 28, 2023, at Mohammed Akile Memorial General Hospital, in Afar Regional State, district of Amibara located in the town of Berta. The finding revealed that 19.8% prevalence among symptomatic children with 61.04% and 38.96% of P. falciparum and P. vivax, respectively. In the present study area, malaria infection in children under five years of age is significantly associated with the presence of stagnant water in the residential area, inappropriate or no use of insecticide-treated net, and indoor residual spraying (IRS). The prevalence of malaria among symptomatic children under five years of age is higher compared to the national prevalence of malaria among the general population. Therefore, community mobilization through health promotion, aiming to interrupt the transmission of malaria at the community level, is paramount.

Comorbidities that involve infectious and noninfectious diseases, such as malaria and cancer, have been described. Cancer and malaria induce changes in the nociceptive and inflammatory responses through similar pathophysiological mechanisms. However, it is unclear whether malaria and antimalarial treatment can change the inflammatory and nociceptive responses induced by solid cancer. Therefore, the present study experimentally evaluated the effect of infection by Plasmodium berghei strain ANKA and chloroquine treatment on the nociceptive and inflammatory responses induced by the solid Ehrlich tumor in male BALB/c mice. On the 1^st experimental day, mice were infected with Plasmodium berghei and injected with tumor cells in the left hind paw. From the 7^th to the 9^th experimental day, mice were treated daily with chloroquine. The parasitemia was evaluated on the 7^th and 10^th days after infection. On the 11^th experimental day, mice were evaluated on the von Frey filament test, the hot plate test, and the paw volume test. At the end of the experimental tests on the 11th day, the peripheral blood of all mice was collected for dosing of IL-1β and TNF-α. The blood parasitemia significantly increased from the 7^th to the 10^th day. The chloroquine treatment significantly decreased the parasitemia on the 10^th day. The presence of the tumor did not significantly change the parasitemia on the 7^th and 10^th days in mice treated and nontreated with chloroquine. On the 11^th day, the mechanical and thermal nociceptive responses significantly increased in mice with tumors. The treatment with antimalarial significantly reduced the mechanical nociceptive response induced by tumors. The hyperalgesia induced by tumors did not change with malaria. The mechanical and thermal hyperalgesia induced by the tumor was significantly reduced in mice treated and healed from malaria. On the 11^th day, the volume of the paw injected by the tumor was significantly increased. The mice treated with chloroquine, infected with malaria, or healed of malaria showed reduced paw edema induced by the tumor. Mice with tumors did not show a change in IL-β and TNF-α serum levels. Mice with tumors showed a significant increase in serum levels of IL-1β but not TNF-α when treated with chloroquine, infected with malaria, or healed of malaria. In conclusion, the results show that malaria infection and chloroquine treatment can influence, in synergic form, the nociceptive and inflammatory responses induced by the solid tumor. Moreover, the mechanical antinociception, the thermal hyperalgesia, and the antiedema effect observed in mice treated with chloroquine and healed from malaria can be related to the increase in the serum level of IL-1β.

Mexican Afro-descendant is a population poorly studied in many aspects, between them the infectious diseases that they suffer. This population is mainly found in the country’s Pacific (Oaxaca and Guerrero states) and Atlantic (Veracruz) coast. In these regions, a diversity of triatomine vectors of the Chagas disease is found. Also, all the genotypes of Trypanosoma cruzi DTUs have been reported. That is why the present study aimed to study the presence of antibodies against T. cruzi and cardiac pathology associated with the Chagas disease in the Mexican Afro-descendant population of Guerrero and Oaxaca. ELISA, Western blot, and recombinant antigen’s ELISA were used to evaluate the seropositivity of these communities. Furthermore, an electrocardiographic study and evaluation of risk factors associated with T. cruzi infection in the Oaxaca and Guerrero populations were conducted. 26.77% of the analyzed population was positive for two serological tests. These percentages are higher than the previously reported for the mestizo population in similar studies. Electrocardiographic results showed cardiac disorder associated with the Chagas disease in the population. Also, risk factors were identified associated with the men’s activities in the outdoor working areas.